Ecological and physiological analysis of immune reactions of <i>Pelophylax ridibundus</i> and <i>P. lessonae</i> (Amphibia: Ranidae) in anthropogeneously transformed territories

The purpose of the work is to assess state Anura populations living in an anthropo-genic territory according a set indicators body’s immune homeostasis. objects study were Pelophylax ridibundus (Pallas, 1771) and P. lessonae (Camerano, 1882), natural reservoirs Nizhny Novgorod. Priority chemical pollutants water bodies determined by spectrophotometry. Species identified using multiplex PCR test system. identification results confirmed sequencing mitochondrial ND2 gene fragment SAI protein. We counted numbers erythrocytes leukocytes, leukocyte profile level complexes all frogs. An excess quality standard for content heavy metals was revealed bodies. Molecular genetic diagnostics showed presence both “pure” individuals with introgressive mtDNA Anatolian form lake frog ( cf. bedriagae ) sample In pond frogs, studied had only species-specific mt- nDNA markers . differed from increased erythrocytes, neutrophils, basophils, small complexes, reduced lymphocytes. changes immunohematological parameters green frogs caused complex henotoxic effect A decrease lymphocytes/eosinophils ratio index shown increase concentration nitrites, activity humoral responses conditions sulfate pollution aquatic environment, proportion myelocytes blood manganese nitrates water. Under environmental stress, regulatory systems reflected stress-induced reaction, which more pronounced body compared ones.